半番鸭胚中鹅细小病毒的分离鉴定及其基因组分子特征分析

doi:10.11843/j.issn.0366-6964.2017.11.016

Abstract

Abstract:

A goose parvovirus (GPV) strain (named MDE) was isolated from mule duck embryos and mule ducks hatched and identified by colloidal gold labeled technique, electron microscope and PCR. The results shows that the virions were spherical with a diameter of approximately 20-24 nm. The colloidal gold test results were that GPV is positive. The results of complete genomic sequences analysis demonstrated that the full-length genome of MDE strain is 5106 bp in length. The nucleotide homology of genomic sequences between MDE strain and other 15 GPV isolates ranged from 93.2% to 98.1%, sharing the greater similarity to strain SH and the lower similarity to strain M15 isolated from mule ducks. Also, the VP1 gene shared higher similarity to the vaccine SYG61v strain. Phylogenetic analysis of VP1 and NS1 gene showed that MDE was a distinct lineage of goose parvovirus but separate from Muscovy duck parvovirus (MDPV). Compared with the vaccine SYG61v strain, MDE add the deduced 703NRTS glycosylation in VP1 protein. In conclusion,The isolated GPV strains in this study can be transmitted vertically by mule duck embryos and enriched the ecology of GPV.

CLC Number:

S852.659.2

FU Qiu-ling, CHENG Long-fei, WAN Chun-he, FU Guang-hua, SHI Shao-hua, CHEN Hong-mei, LIU Rong-chang, CHEN Cui-teng, CHEN Zhen, ZHU Chun-hua, HUANG Yu. Identification and Complete Genomic Sequence Analysis of a Goose Parvovirus from Mule Duck Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2148-2156.

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Identification and Complete Genomic Sequence Analysis of a Goose Parvovirus from Mule Duck Embryos

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